The inhibitor of volume-regulated anion channels DCPIB activates TREK potassium channels in cultured astrocytes.

ABSTRACT:

Background and purpose

The ethacrynic acid derivative, 4-(2-butyl-6,7-dichlor-2-cyclopentylindan-1-on-5-yl) oxobutyric acid (DCPIB) is considered to be a specific and potent inhibitor of volume-regulated anion channels (VRACs). In the CNS, DCPIB was shown to be neuroprotective through mechanisms principally associated to its action on VRACs. We hypothesized that DCPIB could also regulate the activity of other astroglial channels involved in cell volume homeostasis.

Experimental approach

Experiments were performed in rat cortical astrocytes in primary culture and in hippocampal astrocytes in situ. The effect of DCPIB was evaluated by patch-clamp electrophysiology and immunocytochemical techniques. Results were verified by comparative analysis with recombinant channels express

SUBMITTER: Minieri L 

PROVIDER: S-EPMC3594680 | biostudies-literature | 2013 Mar

REPOSITORIES: biostudies-literature