Differential expression of the RANKL/RANK/OPG system is associated with bone metastasis in human non-small cell lung cancer.

ABSTRACT:

Background

Human non-small cell lung cancer (NSCLC) patients exhibit a high propensity to develop skeletal metastasis, resulting in excessive osteolytic activity. The RANKL/RANK/OPG system, which plays a pivotal role in bone remodeling by regulating osteoclast formation and activity, is of potential interest in this context.

Materials and methods

Reverse transcriptase polymerase chain reaction, western blotting, and immunohistochemical analysis were used to examine the expression of RANKL, RANK, and OPG in human NSCLC cell lines with different metastatic potentials, as well as in 52 primary NSCLC samples and 75 NSCLC bone metastasis samples. In primary NSCLC patients, the expression of these proteins was correlated with clinicopathological parameters. Recombinant human RANKL

SUBMITTER: Peng X 

PROVIDER: S-EPMC3596380 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature