Sphingosine and FTY720 are potent inhibitors of the transient receptor potential melastatin 7 (TRPM7) channels.

ABSTRACT:

Background and purpose

Transient receptor potential melastatin 7 (TRPM7) is a unique channel kinase which is crucial for various physiological functions. However, the mechanism by which TRPM7 is gated and modulated is not fully understood. To better understand how modulation of TRPM7 may impact biological processes, we investigated if TRPM7 can be regulated by the phospholipids sphingosine (SPH) and sphingosine-1-phosphate (S1P), two potent bioactive sphingolipids that mediate a variety of physiological functions. Moreover, we also tested the effects of the structural analogues of SPH, N,N-dimethyl-D-erythro-sphingosine (DMS), ceramides and FTY720 on TRPM7.

Experimental approach

HEK293 cells stably expressing TRPM7 were used for whole-cell, single-channel and macropatch curr

SUBMITTER: Qin X 

PROVIDER: S-EPMC3596637 | biostudies-literature | 2013 Mar

REPOSITORIES: biostudies-literature