A real time chemotaxis assay unveils unique migratory profiles amongst different primary murine macrophages.

ABSTRACT: Chemotaxis assays are an invaluable tool for studying the biological activity of inflammatory mediators such as CC chemokines, which have been implicated in a wide range of chronic inflammatory diseases. Conventional chemotaxis systems such as the modified Boyden chamber are limited in terms of the data captured given that the assays are analysed at a single time-point. We report the optimisation and validation of a label-free, real-time cell migration assay based on electrical cell impedance to measure chemotaxis of different primary murine macrophage populations in response to a range of CC chemokines and other chemoattractant signalling molecules. We clearly demonstrate key differences in the migratory behavior of different murine macrophage populations and show that this dynamic system

SUBMITTER: Iqbal AJ 

PROVIDER: S-EPMC3597586 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature