Differential integrity of TALE nuclease genes following adenoviral and lentiviral vector gene transfer into human cells.

ABSTRACT: The array of genome editing strategies based on targeted double-stranded DNA break formation have recently been enriched through the introduction of transcription activator-like type III effector (TALE) nucleases (TALENs). To advance the testing of TALE-based approaches, it will be crucial to deliver these custom-designed proteins not only into transformed cell types but also into more relevant, chromosomally stable, primary cells. Viral vectors are among the most effective gene transfer vehicles. Here, we investigated the capacity of human immunodeficiency virus type 1- and adenovirus-based vectors to package and deliver functional TALEN genes into various human cell types. To this end, we attempted to assemble particles of these two vector classes, each encoding a monomer of a TALEN pair

SUBMITTER: Holkers M 

PROVIDER: S-EPMC3597656 | biostudies-literature | 2013 Mar

REPOSITORIES: biostudies-literature