Validation of an entirely in vitro approach for rapid prototyping of DNA regulatory elements for synthetic biology.

ABSTRACT: A bottleneck in our capacity to rationally and predictably engineer biological systems is the limited number of well-characterized genetic elements from which to build. Current characterization methods are tied to measurements in living systems, the transformation and culturing of which are inherently time-consuming. To address this, we have validated a completely in vitro approach for the characterization of DNA regulatory elements using Escherichia coli extract cell-free systems. Importantly, we demonstrate that characterization in cell-free systems correlates and is reflective of performance in vivo for the most frequently used DNA regulatory elements. Moreover, we devise a rapid and completely in vitro method to generate DNA templates for cell-free systems, bypassing the need for DNA t

SUBMITTER: Chappell J 

PROVIDER: S-EPMC3597704 | biostudies-literature | 2013 Mar

REPOSITORIES: biostudies-literature