Molecular characterization of avian reovirus isolates in Tunisia.

ABSTRACT:

Background

Genotype analyses of avian reoviruses isolated from organ samples collected from chickens with suspicious clinical symptoms, between 1997-2008, was based on sequences for both σC and σB genes and aligned with those published in the Genbank, making it possible to carry out studies of molecular classification and relationships.

Methods

The full length of the known variable protein σC and part of the σB encoding genes, were amplified with RT-PCR, using conserved primers. PCR products were sequenced and the sequences were analyzed and aligned with avian reovirus sequences from the Genbank database.

Results

The sequences of σC-encoding genes of all the isolated strains indicated their close relationship with the American, Chinese and Indian strains. Taking the A

SUBMITTER: Hellal Kort Y 

PROVIDER: S-EPMC3598504 | biostudies-literature | 2013 Jan

REPOSITORIES: biostudies-literature