Validation of reference genes for quantitative real-time PCR studies in the dentate gyrus after experimental febrile seizures.

ABSTRACT:

Background

Quantitative real-time PCR (qPCR) is a commonly used technique to quantify gene expression levels. Validated normalization is essential to obtain reliable qPCR data. In that context, normalizing to multiple reference genes has become the most popular method. However, expression of reference genes may vary per tissue type, developmental stage and in response to experimental treatment. It is therefore imperative to determine stable reference genes for a specific sample set and experimental model. The present study was designed to validate potential reference genes in hippocampal tissue from rats that had experienced early-life febrile seizures (FS). To this end, we applied an established model in which FS were evoked by exposing 10-day old rat pups to heated air. One week

SUBMITTER: Swijsen A 

PROVIDER: S-EPMC3598510 | biostudies-literature | 2012 Dec

REPOSITORIES: biostudies-literature