Transporter engineering for improved tolerance against alkane biofuels in Saccharomyces cerevisiae.

ABSTRACT:

Background

Hydrocarbon alkanes, components of major fossil fuels, are considered as next-generation biofuels because their biological production has recently been shown to be possible. However, high-yield alkane production requires robust host cells that are tolerant against alkanes, which exhibit cytotoxicity. In this study, we aimed to improve alkane tolerance in Saccharomyces cerevisiae, a key industrial microbial host, by harnessing heterologous transporters that potentially pump out alkanes.

Results

To this end, we attempted to exploit ABC transporters in Yarrowia lipolytica based on the observation that it utilizes alkanes as a carbon source. We confirmed the increased transcription of ABC2 and ABC3 transporters upon exposure to a range of alkanes in Y. lipolytica. We

SUBMITTER: Chen B 

PROVIDER: S-EPMC3598725 | biostudies-literature | 2013 Feb

REPOSITORIES: biostudies-literature