Digital gene expression tag profiling analysis of the gene expression patterns regulating the early stage of mouse spermatogenesis.

ABSTRACT: Detailed characterization of the gene expression patterns in spermatogonia and primary spermatocytes is critical to understand the processes which occur prior to meiosis during normal spermatogenesis. The genome-wide expression profiles of mouse type B spermatogonia and primary spermatocytes were investigated using the Solexa/Illumina digital gene expression (DGE) system, a tag based high-throughput transcriptome sequencing method, and the developmental processes which occur during early spermatogenesis were systematically analyzed. Gene expression patterns vary significantly between mouse type B spermatogonia and primary spermatocytes. The functional analysis revealed that genes related to junction assembly, regulation of the actin cytoskeleton and pluripotency were most significantly dif

SUBMITTER: Zhang X 

PROVIDER: S-EPMC3598852 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature