Transcriptional profiling of Saccharomyces cerevisiae exposed to propolis.

ABSTRACT:

Background

Propolis is a natural product of plant resins collected by honeybees (Apis mellifera) from various plant sources. Our previous studies indicated that propolis sensitivity is dependent on the mitochondrial function and that vacuolar acidification and autophagy are important for yeast cell death caused by propolis. Here, we extended our understanding of propolis-mediated cell death in the yeast Saccharomyces cerevisiae by applying systems biology tools to analyze the transcriptional profiling of cells exposed to propolis.

Methods

We have used transcriptional profiling of S. cerevisiae exposed to propolis. We validated our findings by using real-time PCR of selected genes. Systems biology tools (physical protein-protein interaction [PPPI] network) were applied to ana

SUBMITTER: de Castro PA 

PROVIDER: S-EPMC3598864 | biostudies-literature | 2012 Oct

REPOSITORIES: biostudies-literature