Pancreatic cancer circulating tumour cells express a cell motility gene signature that predicts survival after surgery.

ABSTRACT:

Background

Most cancer deaths are caused by metastases, resulting from circulating tumor cells (CTC) that detach from the primary cancer and survive in distant organs. The aim of the present study was to develop a CTC gene signature and to assess its prognostic relevance after surgery for pancreatic ductal adenocarcinoma (PDAC).

Methods

Negative depletion fluorescence activated cell sorting (FACS) was developed and validated with spiking experiments using cancer cell lines in whole human blood samples. This FACS-based method was used to enrich for CTC from the blood of 10 patients who underwent surgery for PDAC. Total RNA was isolated from 4 subgroup samples, i.e. CTC, haematological cells (G), original tumour (T), and non-tumoural pancreatic control tissue (P). After RNA qu

SUBMITTER: Sergeant G 

PROVIDER: S-EPMC3599097 | biostudies-literature | 2012 Nov

REPOSITORIES: biostudies-literature