Genomic variations associated with attenuation in Mycobacterium avium subsp. paratuberculosis vaccine strains.

ABSTRACT:

Background

Mycobacterium avium subspecies paratuberculosis (MAP) whole cell vaccines have been widely used tools in the control of Johne's disease in animals despite being unable to provide complete protection. Current vaccine strains derive from stocks created many decades ago; however their genotypes, underlying mechanisms and relative degree of their attenuation are largely unknown.

Results

Using mouse virulence studies we confirm that MAP vaccine strains 316 F, II and 2e have diverse but clearly attenuated survival and persistence characteristics compared with wild type strains. Using a pan genomic microarray we characterise the genomic variations in a panel of vaccine strains sourced from stocks spanning over 40 years of maintenance. We describe multiple genomic variati

SUBMITTER: Bull TJ 

PROVIDER: S-EPMC3599157 | biostudies-literature | 2013 Jan

REPOSITORIES: biostudies-literature