Expression of a secretory α-glucosidase II from Apis cerana indica in Pichia pastoris and its characterization.

ABSTRACT:

Background

α-glucosidase (HBGase) plays a key role in hydrolyzing α-glucosidic linkages. In Apis mellifera, three isoforms of HBGase (I, II and III) have been reported, which differ in their nucleotide composition, encoding amino acid sequences and enzyme kinetics. Recombinant (r)HBGase II from A. cerana indica (rAciHBGase II) was focused upon here due to the fact it is a native and economic honeybee species in Thailand. The data is compared to the two other isoforms, AciHBGase I and III from the same bee species and to the three isoforms (HBGase I, II and III) in different bee species where available.

Results

The highest transcript expression level of AciHBGase II was found in larvae and pupae, with lower levels in the eggs of A. cerana indica but it was not found in forage

SUBMITTER: Kaewmuangmoon J 

PROVIDER: S-EPMC3599193 | biostudies-literature | 2013 Feb

REPOSITORIES: biostudies-literature