Keratinocyte cytoskeletal roles in cell sheet engineering.

ABSTRACT:

Background

There is an increasing need to understand cell-cell interactions for cell and tissue engineering purposes, such as optimizing cell sheet constructs, as well as for examining adhesion defect diseases. For cell-sheet engineering, one major obstacle to sheet function is that cell sheets in suspension are fragile and, over time, will contract. While the role of the cytoskeleton in maintaining the structure and adhesion of cells cultured on a rigid substrate is well-characterized, a systematic examination of the role played by different components of the cytoskeleton in regulating cell sheet contraction and cohesion in the absence of a substrate has been lacking.

Results

In this study, keratinocytes were cultured until confluent and cell sheets were generated using dis

SUBMITTER: Wei Q 

PROVIDER: S-EPMC3599259 | biostudies-literature | 2013 Feb

REPOSITORIES: biostudies-literature