Dataset Information

Detection of DENV-4 genotype I from mosquitoes collected in the city of Manaus, Brazil.

ABSTRACT:

Background

Dengue epidemics have been reported in Brazil since 1981. In Manaus, a large city in the Amazon region, dengue is endemic with all four-virus serotypes (DENV-1, -2, -3, and -4) simultaneously causing human disease. In 2008, during a surveillance of dengue virus in mosquitoes in the district of Tancredo Neves in Manaus, 260 mosquitoes of Aedes genus were captured, identified and grouped into pools of 10 mosquitoes.

Findings

RNA extracts of mosquito pools were tested by a RT-Hemi-Nested-PCR for detection of flaviviruses. One amplicon of 222 bp, compatible with dengue virus serotype 4, was obtained from a pool of Aedes aegypti. The nucleotide sequence of the amplicon indicated that the mosquitoes were infected with DENV-4 of genotype I. This virus of Asian origin has been described in Manaus in 2008 infecting acute febrile illness patients.

Conclusion

This is the first report of dengue virus serotype 4 genotype I infecting Aedes aegypti in the Americas.

SUBMITTER: Figueiredo ML

PROVIDER: S-EPMC3599326 | biostudies-literature | 2013 Feb

REPOSITORIES: biostudies-literature

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Publications

Detection of DENV-4 genotype I from mosquitoes collected in the city of Manaus, Brazil.

Figueiredo Mario Luis Garcia de ML Alfonso Helda L HL Amarilla Alberto Anastacio AA Figueiredo Luiz Tadeu Moraes LT Aquino Victor Hugo VH Costa Cristóvão Alves da CA Luz Sergio Luiz Bessa SL

Virology journal 20130219

<h4>Background</h4>Dengue epidemics have been reported in Brazil since 1981. In Manaus, a large city in the Amazon region, dengue is endemic with all four-virus serotypes (DENV-1, -2, -3, and -4) simultaneously causing human disease. In 2008, during a surveillance of dengue virus in mosquitoes in the district of Tancredo Neves in Manaus, 260 mosquitoes of Aedes genus were captured, identified and grouped into pools of 10 mosquitoes.<h4>Findings</h4>RNA extracts of mosquito pools were tested by a ...[more]

PMID: 23421733

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Dataset Information

Detection of DENV-4 genotype I from mosquitoes collected in the city of Manaus, Brazil.

Background

Findings

Conclusion

Publications

Detection of DENV-4 genotype I from mosquitoes collected in the city of Manaus, Brazil.

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