High glucose induces apoptosis and suppresses proliferation of adult rat neural stem cells following in vitro ischemia.

ABSTRACT:

Background

Post-stroke hyperglycemia appears to be associated with poor outcome from stroke, greater mortality, and reduced functional recovery. Focal cerebral ischemia data support that neural stem cells (NSCs) play an important role in post-ischemic repair. Here we sought to evaluate the negative effects of hyperglycemia on the cellular biology of NSCs following anoxia, and to test whether high glucose affects NSC recovery from ischemic injury.

Results

In this study, we used immortalized adult neural stem cells lines and we induced in vitro ischemia by 6 h oxygen and glucose deprivation (OGD) in an anaerobic incubator. Reperfusion was performed by returning cells to normoxic conditions and the cells were then incubated in experimental medium with various concentrations of

SUBMITTER: Chen J 

PROVIDER: S-EPMC3599336 | biostudies-literature | 2013 Mar

REPOSITORIES: biostudies-literature