Assembling a cellulase cocktail and a cellodextrin transporter into a yeast host for CBP ethanol production.

ABSTRACT:

Background

Many microorganisms possess enzymes that can efficiently degrade lignocellulosic materials, but do not have the capability to produce a large amount of ethanol. Thus, attempts have been made to transform such enzymes into fermentative microbes to serve as hosts for ethanol production. However, an efficient host for a consolidated bioprocess (CBP) remains to be found. For this purpose, a synthetic biology technique that can transform multiple genes into a genome is instrumental. Moreover, a strategy to select cellulases that interact synergistically is needed.

Results

To engineer a yeast for CBP bio-ethanol production, a synthetic biology technique, called "promoter-based gene assembly and simultaneous overexpression" (PGASO), that can simultaneously transform and

SUBMITTER: Chang JJ 

PROVIDER: S-EPMC3599373 | biostudies-literature | 2013 Feb

REPOSITORIES: biostudies-literature