DNA methylation profile dynamics of tissue-dependent and differentially methylated regions during mouse brain development.

ABSTRACT:

Background

Tissues and their component cells have unique DNA methylation profiles comprising DNA methylation patterns of tissue-dependent and differentially methylated regions (T-DMRs). Previous studies reported that DNA methylation plays crucial roles in cell differentiation and development. Here, we investigated the genome-wide DNA methylation profiles of mouse neural progenitors derived from different developmental stages using HpyCH4IV, a methylation-sensitive restriction enzyme that recognizes ACGT residues, which are uniformly distributed across the genome.

Results

Using a microarray-based genome-wide DNA methylation analysis system focusing on 8.5-kb regions around transcription start sites (TSSs), we analyzed the DNA methylation profiles of mouse neurospheres derived

SUBMITTER: Hirabayashi K 

PROVIDER: S-EPMC3599493 | biostudies-literature | 2013 Feb

REPOSITORIES: biostudies-literature