Reverse transcription loop-mediated isothermal amplification assay for rapid detection of Bovine Rotavirus.

ABSTRACT:

Background

Bovine rotavirus (BRV) infection is common in young calves. This viral infection causes acute diarrhea leading to death. Rapid identification of infected calves is essential to control BRV successfully. Therefore development of simple, highly specific, and sensitive detection method for BRV is needed.

Results

A reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay was developed and optimized for rapid detection of BRV. Specific primer sets were designed to target the sequences of the VP6 gene of the neonatal calf diarrhea virus (NCDV) strain of BRV. The RT-LAMP assay was performed in a water bath for 60 minutes at 63°C, and the amplification products were visualized either directly or under ultraviolet light. This BRV specific RT-LAMP assay

SUBMITTER: Xie Z 

PROVIDER: S-EPMC3599620 | biostudies-literature | 2012 Aug

REPOSITORIES: biostudies-literature