Identification and functional analysis of cytochrome P450 complement in Streptomyces virginiae IBL14.

ABSTRACT:

Background

As well known, both natural and synthetic steroidal compounds are powerful endocrine disrupting compounds (EDCs) which can cause reproductive toxicity and affect cellular development in mammals and thus are generally regarded as serious contributors to water pollution. Streptomyces virginiae IBL14 is an effective degradative strain for many steroidal compounds and can also catalyze the C25 hydroxylation of diosgenin, the first-ever biotransformation found on the F-ring of diosgenin.

Results

To completely elucidate the hydroxylation function of cytochrome P450 genes (CYPs) found during biotransformation of steroids by S. virginiae IBL14, the whole genome sequencing of this strain was carried out via 454 Sequencing Systems. The analytical results of BLASTP showed th

SUBMITTER: Li ZZ 

PROVIDER: S-EPMC3599690 | biostudies-literature | 2013 Feb

REPOSITORIES: biostudies-literature