Molecular characterization of measles viruses that circulated in Cameroon between 2010 and 2011.

ABSTRACT:

Background

Measles virus (MeV) is monotypic, but genetic variation in the hemagglutinin H and nucleoprotein N genes can be analyzed by molecular epidemiologic techniques and used to study virus transmission patterns. The World Health Organization currently recognizes 8 clades (A-H) within which are 24 genotypes of MeV and one provisional genotype, d11. Genotype B3 is clearly the endemic genotype in most of African continent where it is widely distributed. We provide an update on the molecular characterization of wild-type MeVs that circulated in Cameroon between 2010 and 2011.

Findings

Viral RNA was extracted directly from samples obtained from clinically diagnosed measles patients using QIAamp viral RNA Mini Kit. Reverse transcription and PCR amplification of 634 nucleotide

SUBMITTER: Demanou M 

PROVIDER: S-EPMC3599851 | biostudies-literature | 2013 Mar

REPOSITORIES: biostudies-literature