Population-genomic variation within RNA viruses of the Western honey bee, Apis mellifera, inferred from deep sequencing.

ABSTRACT:

Background

Deep sequencing of viruses isolated from infected hosts is an efficient way to measure population-genetic variation and can reveal patterns of dispersal and natural selection. In this study, we mined existing Illumina sequence reads to investigate single-nucleotide polymorphisms (SNPs) within two RNA viruses of the Western honey bee (Apis mellifera), deformed wing virus (DWV) and Israel acute paralysis virus (IAPV). All viral RNA was extracted from North American samples of honey bees or, in one case, the ectoparasitic mite Varroa destructor.

Results

Coverage depth was generally lower for IAPV than DWV, and marked gaps in coverage occurred in several narrow regions (< 50 bp) of IAPV. These coverage gaps occurred across sequencing runs and were virtually unchanged

SUBMITTER: Cornman RS 

PROVIDER: S-EPMC3599929 | biostudies-literature | 2013 Mar

REPOSITORIES: biostudies-literature