Analysis and mapping of a 3' coterminal transcription unit derived from human cytomegalovirus open reading frames UL30-UL32.

ABSTRACT:

Background

It has been predicted that the UL31 gene originates from the positive strand of the human cytomegalovirus (HCMV) genome, whereas the UL30 and UL32 genes originate from the complementary strand. Except for the UL32 gene, the transcription of this gene region has not been investigated extensively.

Methods

Northern blotting, cDNA library screening, RACE-PCR,and RT-PCR were used.

Results

At least eight transcripts of the antisense orientation of UL31 were transcribed from the UL30-UL32 region during the late phase of HCMV infection. The 3' coterminus of these transcripts was located within the predicted UL30 gene. The longest 6.0-kb transcript was initiated upstream of the predicted UL32 gene. Other transcripts were derived from the predicted UL30 and UL31 gene

SUBMITTER: Ma Y 

PROVIDER: S-EPMC3600006 | biostudies-literature | 2013 Feb

REPOSITORIES: biostudies-literature