Protein secondary structure determination by constrained single-particle cryo-electron tomography.

ABSTRACT: Cryo-electron microscopy (cryo-EM) is a powerful technique for 3D structure determination of protein complexes by averaging information from individual molecular images. The resolutions that can be achieved with single-particle cryo-EM are frequently limited by inaccuracies in assigning molecular orientations based solely on 2D projection images. Tomographic data collection schemes, however, provide powerful constraints that can be used to more accurately determine molecular orientations necessary for 3D reconstruction. Here, we propose "constrained single-particle tomography" as a general strategy for 3D structure determination in cryo-EM. A key component of our approach is the effective use of images recorded in tilt series to extract high-resolution information and correct for the contr

SUBMITTER: Bartesaghi A 

PROVIDER: S-EPMC3600145 | biostudies-literature | 2012 Dec

REPOSITORIES: biostudies-literature