Validation of new allele-specific real-time PCR system for thiopurine methyltransferase genotyping in Korean population.

ABSTRACT:

Introduction

Thiopurine drugs are metabolized via S-methylation and catalyzed by thiopurine S-methyltransferase (TPMT). Patients with very low TPMT activity are at high risk of fatal bone marrow toxicity when standard doses of thiopurine drugs are administered. TPMT genotyping can predict TPMT activity and is not affected by transfusion or red blood cell defects. Here, we report a new allele-specific real-time polymerase chain reaction (PCR) system for thiopurine methyltransferase genotyping that is validated in Korean population.

Materials and methods

Three major TPMT single-nucleotide polymorphisms (TPMT 2, 3B, and 3C) were genotyped using real-time PCR with the allele-specific primers and probes. Internal positive controls were included in each well, and an automatic inte

SUBMITTER: Kim S 

PROVIDER: S-EPMC3600258 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature