Zebrafish model for allogeneic hematopoietic cell transplantation not requiring preconditioning.

ABSTRACT: Recent work on vertebrate hematopoiesis has uncovered the presence of deeply rooted similarities between fish and mammals at molecular and cellular levels. Although small animal models such as zebrafish are ideally suited for genetic and chemical screens, the study of cellular aspects of hematopoietic development in lower vertebrates is severely hampered by the complex nature of their histocompatibility-determining genes. Hence, even when hosts are sublethally irradiated before hematopoietic cell transplantation, stable and long-term reconstitution by allogeneic stem cells often fails. Here, we describe the unexpected observation that transplantation and maintenance of allogeneic hematopoietic stem cells in zebrafish homozygous for the c-myb(t25127) allele, carrying a missense mutation (Il

SUBMITTER: Hess I 

PROVIDER: S-EPMC3600459 | biostudies-literature | 2013 Mar

REPOSITORIES: biostudies-literature