Statistical method for comparing the level of intracellular organization between cells.

ABSTRACT: Systems level approaches to analyzing complex emergent behavior require quantitative characterization of alterations of behavior on both the microscale and macroscale. Here we consider the problem of cellular organization and describe a statistical methodology for quantitative comparison of the internal organization between different populations of similar physical objects, such as cells. This comparison is achieved with several steps of analysis. Starting with three-dimensional or two-dimensional images of cells, images are segmented to identify individual cells. Locations of internal points of interest, such as organelles or proteins, are recorded. To define the configuration of internal points in each cell, the individual cells are subjected to bounded Voronoi tessellation: subdividing

SUBMITTER: Apte ZS 

PROVIDER: S-EPMC3600484 | biostudies-literature | 2013 Mar

REPOSITORIES: biostudies-literature