Cultured porcine trabecular meshwork cells display altered lysosomal function when subjected to chronic oxidative stress.

ABSTRACT:

Purpose

To investigate the effects of chronic oxidative stress on lysosomal function in trabecular meshwork (TM) cells.

Methods

Confluent cultures of porcine TM cells were grown for 2 weeks in physiological (5% O(2)) or hyperoxic conditions (40% O(2)) in the presence or absence of the protease inhibitor leupeptin (10 microM). The following parameters were quantified using the fluorogenic probes indicated within parentheses: autofluorescence, intracellular reactive oxygen species (ROS; H(2)DCFDA), mitochondrial membrane potential (JC-1), mitochondrial content (Mitotracker Red; Invitrogen-Molecular Probes, Eugene, OR), lysosomal content (acridine orange and Lysotracker Red [Invitrogen-Molecular Probes]), autophagic vacuole content (MDC), SA-beta-galactosidase (FDG), and cathep

SUBMITTER: Liton PB 

PROVIDER: S-EPMC3601374 | biostudies-literature | 2008 Sep

REPOSITORIES: biostudies-literature