Ionizing radiation-induced γ-H2AX activity in whole blood culture and the risk of lung cancer.

ABSTRACT:

Background

Phenotypic biomarkers of DNA damage repair may enhance cancer risk prediction. The γ-H2AX formed at the sites of double-strand break (DSB) after ionizing radiation is a specific marker of DNA damage.

Methods

In an ongoing case-control study, the baseline and ionizing radiation-induced γ-H2AX levels in peripheral blood lymphocytes (PBL) from frequency-matched 306 untreated patients with lung cancer and 306 controls were measured by a laser scanning cytometer-based immunocytochemical method. The ratio of ionizing radiation-induced γ-H2AX level to the baseline was used to evaluate interindividual variation of DSB damage response and to assess the risk of lung cancer by using unconditional multivariable logistic regression with adjustment of age, sex, ethnicity, smoki

SUBMITTER: He Y 

PROVIDER: S-EPMC3601549 | biostudies-literature | 2013 Mar

REPOSITORIES: biostudies-literature