Proteome-wide mapping of cholesterol-interacting proteins in mammalian cells.

ABSTRACT: Cholesterol is an essential structural component of cellular membranes and serves as a precursor for several classes of signaling molecules. Cholesterol exerts its effects and is, itself, regulated in large part by engagement in specific interactions with proteins. The full complement of sterol-binding proteins that exist in mammalian cells, however, remains unknown. Here we describe a chemoproteomic strategy that uses clickable, photoreactive sterol probes in combination with quantitative mass spectrometry to globally map cholesterol-protein interactions directly in living cells. We identified over 250 cholesterol-binding proteins, including receptors, channels and enzymes involved in many established and previously unreported interactions. Prominent among the newly identified interacting

SUBMITTER: Hulce JJ 

PROVIDER: S-EPMC3601559 | biostudies-literature | 2013 Mar

REPOSITORIES: biostudies-literature