A triad interaction in the fingers subdomain of DNA polymerase beta controls polymerase activity.

ABSTRACT: DNA polymerase beta (pol beta) is the main polymerase involved in the base excision repair pathway responsible for repairing damaged bases in the DNA. Previous studies on the H285D mutant of pol beta suggested that the C-terminal region of the polymerase is important for polymerase function. In this study, the C-terminal region of pol beta was mutated to assess its role in polymerization. Kinetic experiments showed that the C-terminal region is required for wild-type polymerase activity. Additionally, an interaction between the fingers and palm subdomain revealed itself to be required for polymerase activity. The E316R mutant of pol beta was shown to have a 29,000-fold reduction in polymerization rate with no reduction in nucleotide binding, suggesting that there exists a noncovalent mecha

SUBMITTER: Murphy DL 

PROVIDER: S-EPMC3601662 | biostudies-literature | 2011 Apr

REPOSITORIES: biostudies-literature