Impact of viral attachment factor expression on antibody-mediated neutralization of flaviviruses.

ABSTRACT: Neutralization of flaviviruses requires engagement of the virion by antibodies with a stoichiometry that exceeds a required threshold. Factors that modulate the number of antibodies bound to an individual virion when it contacts target cells impact neutralization potency. However, the contribution of cellular factors to the potency of neutralizing antibodies has not been explored systematically. Here we investigate the relationship between expression level of a viral attachment factor on cells and the neutralizing potency of antibodies. Analysis of the attachment factor DC-SIGNR on cells in neutralization studies failed to identify a correlation between DC-SIGNR expression and antibody-mediated protection. Furthermore, neutralization potency was equivalent on a novel Jurkat cell line induc

SUBMITTER: Obara CJ 

PROVIDER: S-EPMC3601793 | biostudies-literature | 2013 Mar

REPOSITORIES: biostudies-literature