Dataset Information
Nedd8 processing enzymes in Schizosaccharomyces pombe.
Ontology highlight
ABSTRACT: Background
Conjugation of the ubiquitin-like modifier Nedd8 to cullins is critical for the function of SCF-type ubiquitin ligases and thus facilitates ubiquitin conjugation and ultimately degradation of SCF substrates, including several cell cycle regulators. Like ubiquitin, Nedd8 is produced as a precursor that must first be processed before it becomes active. In Saccharomyces cerevisiae this is carried out exclusively by the enzyme Yuh1.Results
Here we show that in the fission yeast, Schizosaccharomyces pombe, the Yuh1 orthologue, Uch1, is not the sole Nedd8 processing enzyme. Instead it appears that deubiquitylating enzymes can efficiently process the Nedd8 precursor in vivo.Conclusions
Several enzymes contribute to Nedd8 precursor processing including a number of deubiquitylating enzymes.
SUBMITTER: O'Donoghue JE
PROVIDER: S-EPMC3602023 | biostudies-literature | 2013 Mar
REPOSITORIES: biostudies-literature
Publications
Nedd8 processing enzymes in Schizosaccharomyces pombe.
BMC biochemistry 20130315
<h4>Background</h4>Conjugation of the ubiquitin-like modifier Nedd8 to cullins is critical for the function of SCF-type ubiquitin ligases and thus facilitates ubiquitin conjugation and ultimately degradation of SCF substrates, including several cell cycle regulators. Like ubiquitin, Nedd8 is produced as a precursor that must first be processed before it becomes active. In Saccharomyces cerevisiae this is carried out exclusively by the enzyme Yuh1.<h4>Results</h4>Here we show that in the fission ...[more]