Design of a tobacco exon array with application to investigate the differential cadmium accumulation property in two tobacco varieties.

ABSTRACT:

Background

For decades the tobacco plant has served as a model organism in plant biology to answer fundamental biological questions in the areas of plant development, physiology, and genetics. Due to the lack of sufficient coverage of genomic sequences, however, none of the expressed sequence tag (EST)-based chips developed to date cover gene expression from the whole genome. The availability of Tobacco Genome Initiative (TGI) sequences provides a useful resource to build a whole genome exon array, even if the assembled sequences are highly fragmented. Here, the design of a Tobacco Exon Array is reported and an application to improve the understanding of genes regulated by cadmium (Cd) in tobacco is described.

Results

From the analysis and annotation of the 1,271,256 Nicotia

SUBMITTER: Martin F 

PROVIDER: S-EPMC3602038 | biostudies-literature | 2012 Nov

REPOSITORIES: biostudies-literature