Context-specific protein tyrosine kinase 6 (PTK6) signalling in prostate cancer.

ABSTRACT:

Background

Protein tyrosine kinase 6 (PTK6) is an intracellular tyrosine kinase that is distantly related to SRC family kinases. PTK6 is nuclear in normal prostate epithelia, but nuclear localization is lost in prostate tumours. Increased expression of PTK6 is detected in human prostate cancer, especially at metastatic stages, and in other types of cancers, including breast, colon, head and neck cancers, and serous carcinoma of the ovary.

Materials and methods

Potential novel substrates of PTK6 identified by mass spectrometry were validated in vitro. The significance of PTK6-induced phosphorylation of these substrates was addressed using human prostate cell lines by knockdown of endogenous PTK6 or overexpression of targeted PTK6 to different intracellular compartments.

Re

SUBMITTER: Zheng Y 

PROVIDER: S-EPMC3602132 | biostudies-literature | 2013 Apr

REPOSITORIES: biostudies-literature