Contactin 4, -5 and -6 differentially regulate neuritogenesis while they display identical PTPRG binding sites.

ABSTRACT: The neural cell-adhesion molecules contactin 4, contactin 5 and contactin 6 are involved in brain development, and disruptions in contactin genes may confer increased risk for autism spectrum disorders (ASD). We describe a co-culture of rat cortical neurons and HEK293 cells overexpressing and delivering the secreted forms of rat contactin 4-6. We quantified their effects on the length and branching of neurites. Contactin 4-6 effects were different depending on the contactin member and duration of co-culture. At 4 days in culture, contactin 4 and -6 increased the length of neurites, while contactin 5 increased the number of roots. Up to 8 days in culture, contactin 6 progressively increased the length of neurites while contactin 5 was more efficient on neurite branching. We studied the mole

SUBMITTER: Mercati O 

PROVIDER: S-EPMC3603414 | biostudies-literature | 2013 Mar

REPOSITORIES: biostudies-literature