Endothelial cell whole genome expression analysis in a mouse model of early-onset Fuchs' endothelial corneal dystrophy.

ABSTRACT:

Purpose

To investigate the endothelial gene expression profile in a Col8a2 Q455K mutant knock-in mouse model of early-onset Fuchs' endothelial corneal dystrophy (FECD) and identify potential targets that can be correlated to human late-onset FECD.

Methods

Diseased or normal endothelial phenotypes were verified in 12-month-old homozygous Col8a2(Q455K/Q455K) mutant and wild-type mice by clinical confocal microscopy. An endothelial whole genome expression profile was generated by microarray-based analysis. Result validation was performed by real-time PCR. Endothelial COX2 and JUN expression was further studied in human late-onset FECD compared to normal samples.

Results

Microarray analysis demonstrated endothelial expression of 24,538 genes (162 up-regulated and 172 down

SUBMITTER: Matthaei M 

PROVIDER: S-EPMC3604908 | biostudies-literature | 2013 Mar

REPOSITORIES: biostudies-literature