Optimizing heterologous protein production in the periplasm of E. coli by regulating gene expression levels.

ABSTRACT:

Background

In Escherichia coli many heterologous proteins are produced in the periplasm. To direct these proteins to the periplasm, they are equipped with an N-terminal signal sequence so that they can traverse the cytoplasmic membrane via the protein-conducting Sec-translocon. For poorly understood reasons, the production of heterologous secretory proteins is often toxic to the cell thereby limiting yields. To gain insight into the mechanism(s) that underlie this toxicity we produced two secretory heterologous proteins, super folder green fluorescent protein and a single-chain variable antibody fragment, in the Lemo21(DE3) strain. In this strain, the expression intensity of the gene encoding the target protein can be precisely controlled.

Results

Both SFGFP and the single-c

SUBMITTER: Schlegel S 

PROVIDER: S-EPMC3605120 | biostudies-literature | 2013 Mar

REPOSITORIES: biostudies-literature