DNA damage enhances integration of HIV-1 into macrophages by overcoming integrase inhibition.

ABSTRACT:

Background

The prevention of persistent human immunodeficiency virus type 1 (HIV-1) infection requires the clarification of the mode of viral transduction into resting macrophages. Recently, DNA double-strand breaks (DSBs) were shown to enhance infection by D64A virus, which has a defective integrase catalytic activity (IN-CA). However, the mechanism by which DSBs upregulate viral transduction was unclear. Here we analyzed the roles of DSBs during IN-CA-independent viral transduction into macrophages.

Results

We used cellular systems with rare-cutting endonucleases and found that D64A virus integrated efficiently into the sites of artificially induced DSBs. This IN-CA-independent viral transduction was blocked by an inhibitor of ataxia telangiectasia mutated protein (ATM) bu

SUBMITTER: Koyama T 

PROVIDER: S-EPMC3605128 | biostudies-literature | 2013 Feb

REPOSITORIES: biostudies-literature