Construction of a high-quality yeast two-hybrid (Y2H) library and its application in identification of interacting proteins with key vernalization regulator TaVRN-A1 in wheat.

ABSTRACT:

Background

Low temperature is required for the competence of winter wheat to flowering (vernalization), and several key components in the vernalization-mediated flowering pathway have been isolated. A Y2H library is a very useful platform to further unravel novel regulators in the flowering pathway. Thus, there is a necessity to construct a high-quality Y2H library using vernalized winter wheat plants.

Result

We described the construction of a high-quality Y2H library using winter wheat plants with cold-treatment for different weeks to maximize pooling interacting proteins during vernalization. The resultant Y2H library contained ~2.5×10(6) independent clones, with a cell density of ~2.6×10(8) and an average insert size of ~ 1.5 kb. TaVRN-A1 was used as a "bait" to test the

SUBMITTER: Cao S 

PROVIDER: S-EPMC3605349 | biostudies-literature | 2013 Mar

REPOSITORIES: biostudies-literature