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Sediment microbial communities in Great Boiling Spring are controlled by temperature and distinct from water communities.


ABSTRACT: Great Boiling Spring is a large, circumneutral, geothermal spring in the US Great Basin. Twelve samples were collected from water and four different sediment sites on four different dates. Microbial community composition and diversity were assessed by PCR amplification of a portion of the small subunit rRNA gene using a universal primer set followed by pyrosequencing of the V8 region. Analysis of 164 178 quality-filtered pyrotags clearly distinguished sediment and water microbial communities. Water communities were extremely uneven and dominated by the bacterium Thermocrinis. Sediment microbial communities grouped according to temperature and sampling location, with a strong, negative, linear relationship between temperature and richness at all taxonomic levels. Two sediment locations, Sit

SUBMITTER: Cole JK 

PROVIDER: S-EPMC3605714 | biostudies-literature | 2013 Apr

REPOSITORIES: biostudies-literature

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