Breast cancer cell targeting by prenylation inhibitors elucidated in living animals with a bioluminescence reporter.

ABSTRACT:

Purpose

Inhibitors of protein prenylation, including prenyltransferase inhibitors and aminobisphosphonates such as zoledronic acid, are being investigated intensively as therapeutics in cancer and other diseases. Determining whether prenylation inhibitors directly or indirectly target tumor and/or host cells is key to understanding therapeutic mechanisms.

Experimental design

To determine which cell types can be targeted directly by distinct classes of prenylation inhibitors in vivo, we describe herein the development and implementation of a sensitive and pharmacologically specific bioluminescence-based imaging reporter that is inducible by prenylation inhibitors.

Results

In mouse xenograft models of breast cancer, using reporter-bearing mammary fat pad- or bone-locali

SUBMITTER: Chinault SL 

PROVIDER: S-EPMC3605717 | biostudies-literature | 2012 Aug

REPOSITORIES: biostudies-literature