Establishment of immortalized human erythroid progenitor cell lines able to produce enucleated red blood cells.

ABSTRACT: Transfusion of red blood cells (RBCs) is a standard and indispensable therapy in current clinical practice. In vitro production of RBCs offers a potential means to overcome a shortage of transfusable RBCs in some clinical situations and also to provide a source of cells free from possible infection or contamination by microorganisms. Thus, in vitro production of RBCs may become a standard procedure in the future. We previously reported the successful establishment of immortalized mouse erythroid progenitor cell lines that were able to produce mature RBCs very efficiently. Here, we have developed a reliable protocol for establishing immortalized human erythroid progenitor cell lines that are able to produce enucleated RBCs. These immortalized cell lines produce functional hemoglobin and exp

SUBMITTER: Kurita R 

PROVIDER: S-EPMC3606290 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature