First isolation of dengue virus from Lao PDR in a Chinese traveler.

ABSTRACT:

Background

Epidemic dengue activity has been demonstrated in several southern regions of China, but not in Yunnan province, which borders countries in Southeast Asia where dengue is endemic. Many dengue cases imported from Southeast Asia to Yunnan have been reported, but dengue virus (DENV) has not been isolated from any patients. This study is the first to report the isolation of DENV from a Chinese traveler returning to Yunnan from Lao PDR.

Findings

A serum sample was collected from a patient presenting with a febrile illness who returned from Lao PDR in 2009 and was used to inoculate Aedes albopictus C6/36 cells for viral isolation. The viral isolate was identified using reverse transcription-polymerase chain reaction, and phylogenetic analyses based on the full E sequenc

SUBMITTER: Guo X 

PROVIDER: S-EPMC3606315 | biostudies-literature | 2013 Mar

REPOSITORIES: biostudies-literature