Differential denaturation of serum proteome reveals a significant amount of hidden information in complex mixtures of proteins.

ABSTRACT:

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Recently developed proteomic technologies allow to profile thousands of proteins within a high-throughput approach towards biomarker discovery, although results are not as satisfactory as expected. In the present study we demonstrate that serum proteome denaturation is a key underestimated feature; in fact, a new differential denaturation protocol better discriminates serum proteins according to their electrophoretic mobility as compared to single-denaturation protocols. Sixty nine different denaturation treatments were tested and the 3 most discriminating ones were selected (TRIDENT analysis) and applied to human sera, showing a significant improvement of serum protein discrimination as confirmed by MALDI-TOF/MS and LC-MS/MS identification, depending on the type of dena

SUBMITTER: Verdoliva V 

PROVIDER: S-EPMC3606341 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature