The host-range, genomics and proteomics of Escherichia coli O157:H7 bacteriophage rV5.

ABSTRACT:

Background

Bacteriophages (phages) have been used extensively as analytical tools to type bacterial cultures and recently for control of zoonotic foodborne pathogens in foods and in animal reservoirs.

Methods

We examined the host range, morphology, genome and proteome of the lytic E. coli O157 phage rV5, derived from phage V5, which is a member of an Escherichia coli O157:H7 phage typing set.

Results

Phage rV5 is a member of the Myoviridae family possessing an icosahedral head of 91 nm between opposite apices. The extended tail measures 121 x 17 nm and has a sheath of 44 x 20 nm and a 7 nm-wide core in the contracted state. It possesses a 137,947 bp genome (43.6 mol%GC) which encodes 233 ORFs and six tRNAs. Until recently this virus appeared to be phylogenetically iso

SUBMITTER: Kropinski AM 

PROVIDER: S-EPMC3606486 | biostudies-literature | 2013 Mar

REPOSITORIES: biostudies-literature