Identification of differentially expressed genes from Trichoderma harzianum during growth on cell wall of Fusarium solani as a tool for biotechnological application.

ABSTRACT:

Background

The species of T. harzianum are well known for their biocontrol activity against many plant pathogens. However, there is a lack of studies concerning its use as a biological control agent against F. solani, a pathogen involved in several crop diseases. In this study, we have used subtractive library hybridization (SSH) and quantitative real-time PCR (RT-qPCR) techniques in order to explore changes in T. harzianum genes expression during growth on cell wall of F. solani (FSCW) or glucose. RT-qPCR was also used to examine the regulation of 18 genes, potentially involved in biocontrol, during confrontation between T. harzianum and F. solani.

Results

Data obtained from two subtractive libraries were compared after annotation using the Blast2GO suite. A total of 417 an

SUBMITTER: Vieira PM 

PROVIDER: S-EPMC3606605 | biostudies-literature | 2013 Mar

REPOSITORIES: biostudies-literature