Homologous recombination-mediated gene targeting in the liverwort Marchantia polymorpha L.

ABSTRACT: The liverwort Marchantia polymorpha is an emerging model organism on account of its ideal characteristics for molecular genetics in addition to occupying a crucial position in the evolution of land plants. Here we describe a method for gene targeting by applying a positive/negative selection system for reduction of non-homologous random integration to an efficient Agrobacterium-mediated transformation system using M. polymorpha sporelings. The targeting efficiency was evaluated by knocking out the NOP1 gene, which impaired air-chamber formation. Homologous recombination was observed in about 2% of the thalli that passed the positive/negative selection. With the advantage of utilizing the haploid gametophytic generation, this strategy should facilitate further molecular genetic analysis of

SUBMITTER: Ishizaki K 

PROVIDER: S-EPMC3607118 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature