Computational method for calculating fluorescence intensities within three-dimensional structures in cells.

ABSTRACT: The use of fluorescence microscopy is central to cell biology in general, and essential to many fields (e.g., membrane traffic) that rely upon it to identify cellular locations of molecules under study and the extent to which they co-localize with others. Rigorous localization or co-localization data require quantitative image analyses that can vary widely between fields and laboratories. While most published data use two-dimensional images, there is an increasing appreciation for the advantages of collecting three-dimensional data sets. These include the ability to evaluate the entire cell and avoidance of focal plane bias. This is particularly important when imaging and quantifying changes in organelles with irregular borders and which vary in appearance between cells in a population, e.

SUBMITTER: Caster AH 

PROVIDER: S-EPMC3607619 | biostudies-literature | 2012 Oct

REPOSITORIES: biostudies-literature